Sains Malaysiana 54(1)(2025): 9-20

http://doi.org/10.17576/jsm-2025-5401-02

Effect of Combined Prebiotics from Different Sources and Microorganism Mix on Growth Performance of Milkfish, Chanos chanos Forsskal, 1775

(Kesan Gabungan Prebiotik daripada Sumber Berbeza dan Campuran Mikroorganisma terhadap Prestasi Pertumbuhan Ikan Bandeng, Chanos chanos Forsskal, 1775)

SITI ASLAMYAH^1,*, ZAINUDDIN¹ & AYMAN ALAMEEN MOHAMMED²

¹Fisheries Department, Faculty of Marine Science and Fisheries, Hasanuddin University, Jl. Perintis Kemerdekaan No 10, Tamalanrea Indah, 90245, Makassar, Indonesia
²Department of Fisheries and Wildlife Science, College of Animal Production Science and Technology, Sudan University of Science and Technology, Wehda street, 12217, Khartoum, Sudan

Diserahkan: 1 Oktober 2023/Diterima: 5 November 2024

Abstract

This study was conducted to determine the effect of using synbiotics in artificial feed on growth, digestive enzyme activities, glycogen levels, hepatosomatic index, body chemical composition, nutrient retention, gastric emptying rate, and blood glucose levels of milkfish. Milkfish (4.78 ± 0.16 g) were stocked at density of 20 fish per aquarium (50 × 40 × 35 cm³) containing 48 L of water with a salinity of 20 ppt and equipped with a recirculation system. Fish were fed three times a day (7:00 AM, 12:00, and 17:00 PM) at a feeding rate of 5% for 50 days. This study used a completely randomized design (CRD) with five treatments and three replicates. Synbiotics consisting of 10 mL/kg microorganism mix and 2% different sources of prebiotics (sweet potatoes, Kappaphycus alvarezii, green beans, and red onion) were added to the artificial feed. The results indicated that milkfish-fed sweet potatoes and green beans experienced significant growth improvement and enhanced feed efficiency. These prebiotic sources also positively impacted digestive enzyme activities, potentially improving nutrient utilization. Additionally, green beans exhibited the most pronounced effects on liver and muscle glycogen levels, hepatosomatic index, and energy content. Body composition data showed alterations in protein, fat, ash, crude fiber, and nitrogen-free extract percentages due to prebiotic sources. Green beans demonstrated the highest nutrient retention rates. Moreover, the rate of gastric emptying and blood glucose that was observed reached its peak, and the fastest peak decreased in the prebiotic treatment, which was sourced from green beans and sweet potatoes.

Keywords: Artificial feed; growth performance; milkfish; synbiotics

Abstrak

Penyelidikan ini dijalankan untuk menentukan kesan penggunaan sinbiotik dalam makanan buatan terhadap pertumbuhan, aktiviti enzim pencernaan, paras glikogen, indeks hepatosomatik, komposisi kimia badan, pengekalan nutrien, kadar pengosongan gastrik dan paras glukosa darah ikan bandeng. Ikan bandeng (4.78 ± 0.16 g) distok pada ketumpatan 20 ekor setiap akuarium (50 × 40 × 35 cm³) yang mengandungi 48 L air dengan kemasinan 20 ppt dan dilengkapi dengan sistem peredaran semula. Ikan diberi makan tiga kali sehari (7:00 AM, 12:00 dan 17:00 PM) pada kadar pemakanan 5% selama 50 hari. Kajian ini menggunakan reka bentuk rawak sepenuhnya (CRD) dengan lima rawatan dan tiga ulangan. Sinbiotik yang terdiri daripada 10 mL/kg campuran mikroorganisma dan 2% sumber prebiotik yang berbeza (ubi keledek, Kappaphycus alvarezii, kacang hijau dan bawang merah) telah ditambah ke dalam makanan buatan. Keputusan menunjukkan bahawa ikan bandeng yang diberi makan ubi keledek dan kacang hijau mengalami peningkatan pertumbuhan yang ketara dan kecekapan makanan yang dipertingkatkan. Sumber prebiotik ini juga memberi kesan positif kepada aktiviti enzim pencernaan yang berpotensi meningkatkan penggunaan nutrien. Selain itu, kacang hijau menunjukkan kesan yang paling ketara pada tahap glikogen hati dan otot, indeks hepatosomatik dan kandungan tenaga. Data komposisi tubuh menunjukkan perubahan dalam protein, lemak, abu, serat kasar dan peratusan ekstrak bebas nitrogen disebabkan oleh sumber prebiotik. Kacang hijau menunjukkan kadar pengekalan nutrien yang paling tinggi. Selain itu, kadar pengosongan gastrik dan glukosa darah yang diperhatikan mencapai kemuncaknya dan kemuncak terpantas menurun dalam rawatan prebiotik yang diperoleh daripada kacang hijau dan ubi keledek.

Kata kunci: Ikan bandeng; makanan buatan; prestasi pertumbuhan; sinbiotik

RUJUKAN

Abdurrahman, H.Z. & Yanti, Y. 2018. Gambaran umum pengaruh probiotik dan prebiotik pada kualitas daging ayam. TERNAK TROPIKA Journal of Tropical Animal Production 19(2): 95-104. https://doi.org/10.21776/ub.jtapro.2018.019.02.4

Afrizal, A. & Purwanto, A. 2011. Pemanfaatan selulosa bakterial nata de coco sebagai adsorban logam Cu(II) dalam sistem berpelarut air. JRSKT - Jurnal Riset Sains dan Kimia Terapan 1(1): 27-32. https://doi.org/10.21009/jrskt.011.05

Ai, Q., Xu, H., Mai, K., Xu, W., Wang, J. & Zhang, W. 2011. Effects of dietary supplementation of Bacillus subtilis and fructooligosaccharide on growth performance, survival, non-specific immune response and disease resistance of juvenile large yellow croaker, Larimichthys crocea. Aquaculture 317(1-4): 155-161. https://doi.org/10.1016/j.aquaculture.2011.04.036

American Public Health Association (APHA). 1992. Standard Methods for the Examination of Water and Waste Water. 18th ed. Washington DC: American Public Health Association. p. 1268.

Aslamyah, S. 2006. Peningkatan peran mikroba saluran pencernaan untuk memacu pertumbuhan ikan bandeng. Dissertation. Graduate program, Institut Pertanian Bogor, Bogor.

Aslamyah, S., Zainuddin, Z. & Badraeni, B. 2022. The effect of microorganisms combination as probiotics in feed for growth performance, gastric evacuation rates, and blood glucose levels of milkfish, Chanos chanos (Forsskal, 1775). Jurnal Iktiologi Indonesia 22(1): 77-91. https://doi.org/10.32491/jii.v22i1.583

Aslamyah, S., Karim, M.Y. & Badraeni. 2018. Pengaruh dosis mikroorganisme mix. dalam memfermentasi bahan baku pakan yang mengandung Sargassum sp. terhadap kinerja pertumbuhan, komposisi kimia tubuh dan indeks hepatosomatik ikan bandeng, (Chanos chanos Forsskal) Torani Jurnal of Fisheries and Marine Science 1(2): 59-70.

Aslamyah, S., Karim, M.Y. & Badraeni. 2017. Fermentation of seaweed flour with various fermenters to improve the quality of fish feed ingredients. Jurnal Akuakultur Indonesia 16(1): 8-14. https://doi.org/10.19027/jai.16.1.8-14

Association of Official Analytical Chemists (AOAC). 2012. Official Methods of Analysis of the Association of Official Analytical Chemists. 19th ed. Arlington, VA: Association of Official Analytical Chemists.

Bergmeyer, H.U. & Grassi, M. 1983. Methods of Enzymatic Analysis. Volume 2. Weinheim: Verlag Chemie.

Boonanuntanasarn, S., Tiengtam, N., Pitaksong, T., Piromyou, P. & Teaumroong, N. 2018. Effects of dietary inulin and Jerusalem artichoke (Helianthus tuberosus) on intestinal microbiota community and morphology of Nile tilapia (Oreochromis niloticus) fingerlings. Aquaculture Nutrition 24(2): 712-722.

Cerezuela, R., Meseguer, J. & Esteban, M.A. 2011. Current knowledge in synbiotic use for fish aquaculture: A review. Journal of Aquaculture Research and Development S1-008. https://doi.org/10.4172/2155-9546.S1-008

Chen, H.Y. & Durand, J.D. 2016. Advances in milkfish biology and culture in Taiwan. Reviews in Aquaculture 8(3): 187-220.

Craig, P.M. & Moon, T.W. 2013. Methionine restriction affects the phenotypic and transcriptional response of rainbow trout (Oncorhynchus mykiss) to carbohydrate-enriched diets. British Journal of Nutrition 109(3): 402-412.  https://doi.org/10.1017/S0007114512001663

Das, S., Mondal, K. & Haque, S. 2017. A review on application of probiotic, prebiotic and synbiotic for sustainable development of aquaculture. Journal of Entomology and Zoology Studies 5(2): 422-429.

Davani-Davari, D., Negahdaripour, M., Karimzadeh, I., Seifan, M., Mohkam, M., Masoumi, S.J., Berenjian, A. & Ghasemi, Y. 2019. Prebiotics: Definition, types, sources, mechanisms, and clinical applications. Foods 8(3): 92. https://doi.org/10.3390/foods8030092

Djauhari, R., Widanarni, S., Suprayudi, M.A. & Zairin, M. 2017. Growth performance and health status of common carp (Cyprinus carpio) supplemented with prebiotic from sweet potato (Ipomoea batatas) extract. Pakistan Journal of Nutrition 16: 155-163. doi:10.3923/pjn.2017.155.163

Dossou, S., Hossain, M.A., Haque, M.M. & Islam, M.A. 2018. Economic analysis of small-scale shrimp farming in Bangladesh: A case study in Barguna district. Aquaculture Reports 10: 1-7.

El Basuini, M.F., El-Haroun, E.R., Attia, E.I. & Shalaby, S.M. 2017. Evaluation of dietary lipid sources for sea bass (Dicentrarchus labrax) fingerlings: Growth, feed utilization, tissue composition, and histology. Aquaculture 473: 304-311.

Hai, N.V. 2015. The use of probiotics in aquaculture. Journal of Applied Microbiology 119(4): 917-935. https://doi.org/10.1111/jam.12886

Halver, J.E. 1989. Fish Nutrition. 2nd ed. San Diego: Academic Press.

Harikrishnan, R., Devi, G., Balamurugan, P., Abdel-Warith, A.W.A., Younis, E.M., Van Doan, H. & El-Haroun, E. 2023. Immunostimulatory effect of mannan-oligosaccharides supplementation diet in milkfish (Chanos chanos). Fish & Shellfish Immunology 2023: 108568. https://doi.org/10.1016/j.fsi.2023.108568

Lee, Y.C., Lu, Y.H., Lee, J.M., Schafferer, C., Yeh, C.Y., Chu, T.W. & Huang, Y.W. 2022. A production economic analysis of different stocking density and fry size combinations of milkfish, Chanos chanos, farming in Taiwan. Journal of the World Aquaculture Society 53(2): 424-451. https://doi.org/10.1111/jwas.12842

Lesmanawati, W., Widanarni, W., Sukenda, S. & Purbiantoro, W. 2013. The potential of sweet potato oligosaccharide extract as aquaculture probiotic bacteria prebiotic. Jurnal Sains Terapan 3(1): 16-20. https://doi.org/10.29244/jstsv.3.1.16-20

Leung, L.Y. & Woo, N.Y.S. 2012. Influence of dietary carbohydrate level on endocrine status and hepatic carbohydrate metabolism in the marine fish Sparus sarba. Fish Physiology and Biochemistry 38(2): 543-554. https://doi.org/10.1007/s10695-011-9534-8

Marlida, R., Suprayudi, M.A., Widanarni, & Harris, E. 2014. Isolation, selection and application of probiotic bacteria for improvement the growth performance of Humback Groupers (Cromileptes altivelis). International Journal of Science: Basic and Applied Research 16(1): 367-379.

Marte, C.L. 2010. Milkfish aquaculture in the Philippines: An overview. In Milkfish Aquaculture in Asia, edited by Liao, I.C. & Leaño, E.M. Keelung, Taiwan: National Taiwan Ocean University, The Fisheries Society of Taiwan, Asian Fisheries Society and World Aquaculture Society. pp. 33-46.

Masriah, A. 2020. Penambahan limbah cairan rumen sapi pada berbagai level karbohidrat dalam pakan terhadap retensi nutrient dan komposisi kimia tubuh ikan bandeng (Chanos chanos Forsskal). Octopus: Jurnal Ilmu Perikanan 9(1): 33-38.

Merrifield, D.L., Dimitroglou, A., Foey, A., Davies, S.J., Baker, R.T.M., Bøgwald, J., Castex, M. & Ringø, E. 2010. The current status and future focus of probiotic and prebiotic applications for salmonids. Aquaculture 302(1-2): 1-18.  https://doi.org/10.1016/j.aquaculture.2010.02.007

Miller, G.L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry 31(3): 426-428.

Mohammadi, G., Rafiee, G., El Basuini, M.F., Abdel-Latif, H.M.R. & Dawood, M.A.O. 2020. The growth performance, antioxidant capacity, immunological responses, and the resistance against Aeromonas hydrophila in Nile tilapia (Oreochromis niloticus) fed Pistacia vera hulls derived polysaccharide. Fish & Shellfish Immunology 106: 36-43.

Mustakin, F. & Tahir, M.M. 2019. Analisis kandungan glikogen pada hati, otot, dan otak hewan. Canrea Journal 2(2): 75-80.

Mutmainnah, N. 2019. The effect of dissolved glucose on survival rate and performance of swimming crab larvae Portunus pelagicus from zoea stadia to megalopa. International Journal of Fisheries and Aquatic Studies 7(6): 30.

Pollock, C.J. & Jones, T. 1979. Seasonal patterns of fructan metabolism in forage grasses. New Phytologist 83(1): 9-15.

Puri, P., Sharma, J.G. & Singh, R. 2022. Biotherapeutic microbial supplementation for ameliorating fish health: developing trends in probiotics, prebiotics, and synbiotics use in finfish aquaculture. Animal Health Research Reviews 23(2): 113-135. https://doi.org/10.1017/S1466252321000165

Qaddoori, M.S., Najim, S.M. & Al-Niaeem, K.S. 2022. Effects of some probiotics and synbiotic dietary supplementation on growth performance and digestive enzymes activity of common carp, Cyprinus Carpio. Journal of Pharmaceutical Negative Results 13(7): 175-184. https://doi.org/10.47750/pnr.2022.13.S07.027

Reverter, M., Tapissier-Bontemps, N., Sarter, S., Sasal, P. & Caruso, D. 2021. Moving towards more sustainable aquaculture practices: A meta-analysis on the potential of plant-enriched diets to improve fish growth, immunity and disease resistance. Reviews in Aquaculture 13(1): 537-555. https://doi.org/10.1111/raq.12485

Ringø, E. & Song, S.K. 2016. Application of dietary supplements (synbiotics and probiotics in combination with plant products and β-glucans) in aquaculture. Aquaculture Nutrition 22(1): 4-24. https://doi.org/10.1111/anu.12349

Soto, C., Ibáñez, A.L. & Godoy, F.A. 2015. Functional feed in aquaculture: A review. Revista MVZ Córdoba 20(2): 4879-4890.

Takeuchi, T. 1988. Laboratory work chemical evaluation of dietary nutrients. In Fish Nutrition and Mariculture, edited by Watanabe, T. Department of Aquatic Bioscience, Tokyo University of Fisheries. JICA. pp. 179-226.

Tang, Y., Wang, J. & Pan, L. 2020. Effect of prebiotics and synbiotics on intestinal microbiota and metabolic biomarkers in ruminants: A systematic review and meta-analysis. Journal of Animal Science and Biotechnology 11(1): 1-14.

Wang, T., Wu, H.X., Li, W.J., Xu, R., Qiao, F., Du, Z.Y. & Zhang, M.L. 2022. Effects of dietary mannan oligosaccharides (MOS) supplementation on metabolism, inflammatory response and gut microbiota of juvenile Nile tilapia (Oreochromis niloticus) fed with high carbohydrate diet. Fish & Shellfish Immunology 130: 550-559.

Wedemeyer, G.A. & Yasutake, W.T. 1977. Clinical Methods for the Assessment of the Effects of Environmental Stress on Fish Health.  Technical Paper of the US Fish and Wildlife Service.  Volume 89.  USA Washington DC: US Department of the Interior Fish and Wildlife Service.

Wendelaar, S.E. 1997. The stress response in fish. Physiological Reviews 77(3): 591-625. https://doi.org/10.1152/physrev.1997.77.3.591

Yang, P., Yang, W., He, M., Li, X. & Leng, X.J. 2020. Dietary synbiotics improved the growth, feed utilization and intestinal structure of largemouth bass (Micropterus salmoides) juvenile. Aquaculture Nutrition 26(2): 590-600. https://doi.org/10.1111/anu.13020

Zonneveld, N., Huisman, E.A. & Boon, J.H. 1991. Principles of Fish Farming. Jakarta: PT. Gramedia Pustaka Utama. p. 318.

*Pengarang untuk surat-menyurat; email: siti.aslamyah@unhas.ac.id